UMSI 2000 Annual Report: Christopher N. Honda, Principal Investigator Previous Page  |  Table of Contents  |  Next Page

Christopher N. Honda, Principal Investigator


Three-Dimensional Analysis of Spinal Cord Tissue

This project is working to determine spatial relationships between opioid-receptor containing axon terminals of sensory neurons and projection neurons in the spinal cord. Data consists of stacks of images of dual-colored immunofluorescence material collected using a confocal laser scanning microscope. Each stack of images contains a single neuron stained with one fluorophore and axon terminals marked with another.

Research Group

Anie Roche, Research Associate

Before being analyzed further, each stack of images must be processed using deconvolution software (huygens) residing on certain workstations in the Supercomputing Institute's Basic Sciences Computing Laboratory because the deconvolution process is critical to the research.