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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
College of Biological Sciences and Medical School
Department of Genetics, Cell Biology, and Development

PI: Judith G. Berman

Microarray Analysis of Candida albicans Gene Expression

This group studies the human pathogenic fungus Candida albicans. Together with a consortium of seven different research groups, they built and use whole-genome microarrays. The Berman laboratory was responsible for deciding which primers to design for amplification of fragments representing virtually all the open reading frames (ORFs) in the organism. They have used these primers to generate over 7,000 different amplified ORFs, which have been printed to glass slide arrays. The arrays have been used to generate transcription profiles as well as to analyze the DNA content of clinical and laboratory strains that have undergone genome changes using Comparative Genome Hybridization (CGH). CGH has revealed a number of aneuploidies in strains, and especially in strains resistant to specific anti-fungal drugs. The group is also performing analysis of these datasets, along with performing large-scale analysis of global expression patterns in all microarray experiments performed to date by the many laboratories working with Candida albicans and comparing those studies to global analyses of similar work done in the model yeast Saccharomyces cerevisiae.

Group Members

Darren Abbey, Graduate Student
Kelly Bouchonville, Graduate Student
David Bruck, Undergraduate Student
Laura Burrack, Post-doctoral Fellow
Keely Dulmage, Staff
Carrie Ketel, Research Associate
Amnon Koren, Research Associate
Tamar Lahav, Research Associate
Mark McClellan, Staff
Maryam Nejad, Staff
Thomas Ringstrom, Undergraduate Student
Karen Tang, Supercomputing Institute Research Scholar
Hung-Ji Tsai, Graduate Student
Aaron Quick, Staff
Shelly Applen, Undergraduate Student