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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
Institute of Technology
Department of Chemical Engineering and Materials Science

PI: Wei-Shou Hu, Associate Fellow

Genomic and Proteomic Analysis of Cells and Tissues

These researchers are involved in several projects using the Supercomputing Institute laboratories. In one, they are using genomics and proteomics to investigate recombinant Chinese hamster ovary cells. These industrially important cells carry many desirable phenotypic traits, but the alterations at the genomic level that confer those traits are not well understood. The researchers are seeking a better understanding of those key genetic alterations, which will facilitate the development of new cells lines and new processes.

A second project concerns biosynthesis in Streptomyces, which are important microorganisms because they produce more than two-thirds of clinically used antibiotics, as well as other bioactive molecules. The goal of the project is to identify the possible positions of important genes on the regulatory hierarchy of secondary metabolism for these organisms, which would serve as a model for studying synthesis and regulation of a variety of industrially important natural products.

A third project, begun during this period, uses data mining to analyze cell-culture manufacturing processes. The data archives of manufacturing plants may have valuable information that could enhance process robustness. This data-mining effort will aid in developing effective control strategies to ensure process consistency and high productivity. Other projects begun during this period involve phenotypic and genomic characterization of Streptomyces coelicolor regulatory mutants and sequencing of the curdlan-producing strain Agrobacterium sp. ATCC31749.

Group Members

Marlene Castro-Melchor, Graduate Student
Anushree Chatterjee, Graduate Student
Inseok Doo, Visiting Researcher
Nitya M. Jacob, Graduate Student
Kathryn Johnson, Graduate Student
Anne Kantardjieff, Graduate Student
Huong Le, Graduate Student
Bhanu Mulikutla, Graduate Student
Peter Morrin Nissom, Bioprocessing Technology Center, National University of Singapore, Singapore
Jason Derek Owens, Graduate Student
Shikha Sharma, Graduate Student
Kartik Subramanian, Graduate Student
Siguang Sui, Graduate Student