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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
Medical School
Department of Pediatrics

PI: Theresa A. Laguna

Biomarkers of Acute Lung Injury and Remodeling in Pediatric Cystic Fibrosis

Cystic fibrosis (CF) lung disease is characterized by structural damage to the airways and parenchyma. Chronic inflammation and infection lead to airway injury and remodeling, causing loss of lung function and shortened life expectancy. A non-invasive measurement of airway matrix constituents may provide insight into these processes and has potential as a biomarker of lung injury. Airway matrix constituent concentration profiles (desmosine, collagen, glycosaminoglycans and elastin) may be reflective of ongoing lung injury and remodeling. The hypothesis of this project is that concentration of airway matrix constiuents in urine and sputum: will increase from values obtained during an immediately prior stable period in patients experiencing a pulmonary exacerbation, and will correlate with previously validated measures of lung disease, including inflammatory markers and lung function.

The goals of this project are: to determine natural change and variability in airway matrix constituent concentration in a cohort of CF patients during times of clinical stability and during pulmonary exacerbation; to determine if airway matrix constituent concentration can differentiate between a clinically stable and exacerbated state; and to determine if airway matrix constituent concentration, or change in concentration, correlates with previously validated measures of lung disease. Determination of airway matrix constituent concentration may allow development of a panel of biomarkers to facilitate early medical intervention to help preserve lung function. Proteomics are used to analyze the sputum to detect and measure desmosine as well as to determine the proteomic profile in CF sputum.