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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
Medical School
Department of Surgery

PI: Michael A. Maddaus
Co-PI: Robert A. Kratzke
Co-PI: Jonathan D’Cunha

The Effect of Minimally Invasive Versus Open Surgical Approach on Immune Effector Cell Function

Surgery has been known to be associated with a chemically measurable stress response, and there are outcome differences in patients who undergo different types of surgery or different lengths of surgery. This group’s interest is in the effect of the stress response on the immune effector cells in peripheral blood and if the stress of surgery causes changes in the immune effector cell function and gene expression. The researchers have been able to measure a difference in functional activity of the cells and the next logical question is what drives this. They are looking to see if the stress induced by surgery changes gene expression. They are comparing the differential expression of genes at various points after surgery and before, by using Affymetrix microarray analysis. Once a gene signature has been identified, the results will be validated by using quantitative real-time PCR confirmation. If definitive changes are identified, the information would be used for operative planning, prognostic prediction, and risk stratification with implications for future treatment investigations.

Group Members

Shawn S. Groth, Graduate Student
Blake Jacobson, Research Associate
Manish Patel, Research Associate
Natasha Reuth, Graduate Student
Scott Selby, Research Associate
Bryan A. Whitson, Graduate Student