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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
College of Biological Sciences and
College of Food, Agricultural, and Natural Resource Sciences
Department of Plant Biology

PI: M. David Marks

Gene Profiling of Arabidopsis Trichome Development Mutants

The Arabidopsis trichome is a unicellular non-glandular trichome found on rosette leaves, petioles, inflorescences, and sepals. A typical leaf trichome has a stalk with three spike-like branches at the distal end. Mutant alleles for genes that range in function from cytoskeleton elements to transcriptional regulators have been isolated that cause phenotypic alternations to trichomes. These researchers have devised a method to isolate plant trichomes from leaf tissue en masse, allowing for RNA isolation and subsequent microarray analysis. They have obtained transcriptome data in this way from a half-dozen genotypes for the purposes of monitoring what transcriptional perturbations result from the observed phenotypic changes. One of the mutant alleles, the glabra3-shapeshifter (gl3-sst) allele, is a point mutation in a key trancriptional regulator responsible in part for controlling the initiation of trichomes. gl3-sst trichomes expand but are misshapen and retained hallmarks of immature trichomes, thus allowing for the exploration of immature trichome transcriptional profiles.

Group Members

Edward K. Gilding, Graduate Student
Lingtian King, Graduate Student
Jonathan Wenger, Research Associate