Research Abstracts Online
2008 - March 2009
University of Minnesota Twin Cities
Department of Pediatrics
PI: Michael Mauer
Co-PI: Youngki Kim
Microarray Studies of Skin Fibroblasts in Type I Diabetes
Diabetic nephropathy (DN) is the leading cause of kidney failure and was responsible for 46% of all the new cases of kidney failure in the United States in 2004. These researchers are studying cultured skin fibroblast (SF) from type 1 diabetic patients in order to better understand the differences in behavior of these in patients with and without DN. They are testing the hypothesis that there are inherent cellular differences between type 1 diabetic patients with or without DN and that these differences are genetically determined and are associated with altered SF gene expression. They are also using the SF model to study cell behavioral differences in subjects with type 1 diabetes and normal controls to explore cellular behaviors related to the genetics and pathogenesis of this disease. The goal is to use microarray and proteomic techniques to test for gene and protein expression differences in SF from type 1 diabetes patients that have been structurally and functionally polarized into two groups: one a "fast-track” group (high risk of DN) and one a "slow-track” group (low risk of DN). They are also testing whether diabetes per se, compared to controls, influences these cells and whether identical twins discordant for diabetes have similar or different cell behaviors.
The researchers are using the BSCL and the CGL for this project.
Luiza Caramori, Faculty Collaborator
Paul Walker, Research Associate