Research Abstracts Online
2008 - March 2009
University of Minnesota Twin Cities
Department of Medicine
PI: Jeffrey S. Miller
Use of Gene Arrays to Understand the Biology of Human Natural Killer Cells
Despite progress made in the understanding of Natural Killer (NK) cell development, the molecular mechanisms directing differentiation of NK cells and killer-immunoglobulin receptor acquisition during development are poorly understood. This researcher has found that FEZ1 (fasciculation and elongation protein zeta 1), JFC1(C2 domain-containing protein), granzyme M, ATP2A3 (ATPase), CLIC3 (chloride intracellular channel 3), CTL2, PTR4 (peptide-histidine transporter 4), and EDG8 (G-protein-coupled receptor 8) were commonly up-regulated in the more mature NK cell populations and STAT1, NME2 (non-metastatic cells 2), SEC14L1 (SEC14-like 1), RGS16 (regulator of G-protein signaling 16), FUT8 (fucosyltransferase 8) were down-regulated. He used the CGL to study this small list of genes further to understand the final stages of NK cell maturation and elucidate a developmental link between the two best characterized NK cell subsets circulating in blood.