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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
College of Biological Sciences and Medical School
Department of Biochemistry, Molecular Biology, and Biophysics

PI: Douglas H. Ohlendorf, Fellow

Structural Analysis of Macromolecules

The goal of these studies is to understand the structural basis of how macromolecules function. The current focus is on two groups of proteins: dixoygenases that use metal ions to cleave aromatic rings and proteins from gram-positive pathogens. Examples of dioxygenases are protocatechuate 3,4-dioxygenase (PCD), homoprotocatechuate 2,3-dioxygenase (HPCD), and 1,2-catechol dioxygenase (CTD). Examples of proteins from gram-positive pathogens are toxins and proteins involved in the formation of biofilms and in the transfer of antibiotic resistance.

The researchers are refining structures of substrate and inhibitor complexes of mutants of PCD, HPCD, and CTD, and are solving and refining the structures of several proteins from pathogens.

Group Members

Mahati Chintapalli, Supercomputing Institute Undergraduate Intern
Cathleen A. Earhart, Research Associate
Rebecca Hoeft, Graduate Student
Medora Huseby, Undergraduate Student
Andrew Kruse, Undergraduate Student
Ke Shi, Hormel Institute, Austin, Minnesota