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Research Abstracts Online
January 2008 - March 2009

University of Minnesota Twin Cities
College of Food, Agricultural, and Natural Resource Sciences
Department of Agronomy and Plant Genetics

PI: Ronald L. Phillips

Mapping QTLs for Kernel Oil Concentration Using SNP and SSR Markers in Korean High-oil Corn

One way of adding value to corn is by increasing its oil content. Aside from household and other industrial uses, high-oil corn is extensively used as feed because it reduces the amount of fat supplements needed for livestock feed. High-oil corn is also of particular interest because of its potential in the biofuel industry to be a source of both ethanol and biodiesel. The main objective of this study is to characterize a new high-oil corn population from Korea called Korean High Oil (KHO) by conducting a QTL mapping experiment. Based on near-infrared (NIR) and NMR analyses, KHO contains 20% oil in the kernel as opposed to 3.5–4.00 % for normal corn. The mapping population consists of 190 F2 plants derived from multiple crosses between corn inbred lines A619 and KHO. Percent oil was measured using an NIR analyzer on ground kernels bulked from the same ear. DNA from these plants has been extracted and marker polymorphism survey was done using single nucleotide polymorphism (SNP) and simple sequence repeart (SSR) markers. For the SNPs, 60 polymorphic markers were selected out of 220 markers assayed. Genotyping was done using the Sequenom MALDI-TOF SNP genotyping system. On the other hand, 64 polymorphic SSRs were used to genotype the mapping population using 3% agarose gel electrophoresis. A linkage map is currently being constructed using these 124 SNP and SSR markers.

Group Members

Alexander Kahler, Graduate Student
Adrian Stec, Staff
Jayanti Suresh, Staff