Research Abstracts Online
2008 - March 2009
University of Minnesota Twin Cities
Department of Microbiology
PI: Leslie A. Schiff
Endosomal Trafficking Requirements of Reovirus
Viruses enter the cytoplasm by directly penetrating the cell surface or by going through the endocytic pathway. The early endosome stage of the pathway can be reached shortly after cellular binding. As the endosome progresses, the pH decreases with the late endosome having a lower pH (~5.5) than the early endosome (~6.2-6.5). For coated reovirus, proteases found in the endocytic pathway that rely on acidic pH may be important for promoting conformational changes in the capsid proteins. These researchers use siRNA constructs to knock down proteins required for endosome progression, thereby maintaining endosomes at a specific pH. They then determine which parts of the endocytic pathway are necessary for productive reovirus infection.
Wade Schulz, Graduate Student