Research Abstracts Online
2008 - March 2009
University of Minnesota Twin Cities
Department of Neuroscience
PI: Martin W. Wessendorf
Heterodimerization of Opioid Receptors
Pairs of different g-protein coupled receptors have been reported to heterodimerize. By doing so, they should exist within a few nanometers of each other. If these receptors do in fact heterodimerize, it should be possible to use light microscopy to detect single structures in the central nervous system that are labeled for both receptors.
These researchers have been able to detect single structures at the limit of resolution by confocal microscopy (i.e., smaller than 0.3 mm) that are labeled for two different types of receptors. Resolution could in principle be improved by deconvolving the confocal images, however. The researchers use the resources of the BSCL to deconvolve such images. Obtaining higher-resolution images will improve their ability to test which receptors, if any, may exist as heterodimers and to determine their distributions. The researchers are also designing probes that will allow them to localize various receptors using immunocytochemistry and/or in situ hybridization.
Mai Lan Leong, Graduate Student
Steve Schnell, Staff