Research Abstracts Online
January 2009 - March 2010
University of Minnesota Twin Cities
Department of Pediatrics
PI: Bruce R. Blazar
Characterization of the Transgenic Zebrafish tg(SDF-1:dsRed2)
Successful bone marrow transplant depends on the homing and engraftment of transplanted hematopoietic stem cells (HSCs) to the hematopoietic niche in the recipient. The zebrafish Danio rerio is emerging as a new model system for the study of HSC transplant, and is a model organism that offers unparalleled opportunities for in vivo microscopy of the hematopoietic niche during homing and engraftment processes.
Previous work in mammals has shown that the chemokine stromal derived factor 1 (SDF-1) plays a vital role in HSC and progenitor cell homing. It is unknown to what extent the functional roles of SDF-1 have been conserved in the zebrafish. It is hypothesized that in the zebrafish, as in mice and humans, SDF-1 may guide HSCs to the hemotopoietic niche. In initial work, this group has found that in adult zebrafish, SDF-1a is expressed in the hematopoietic kidney. They are using MSI resources to perform image-based characterizations of a line of transgenic zebrafish in expressing the fluorophore dsRed2under control of the endogenous zebrafish SDF-1a promoter. This transgenic line may serve as a foundation for future in vivo investigations of the HSC niche.
Tiffany Glass, Graduate Student
Troy Lund, Research Associate