Research Abstracts Online
January 2009 - March 2010
University of Minnesota Twin Cities
College of Veterinary Medicine
Department of Veterinary Population Medicine
PI: Sagar M. Goyal
Development of an Improved Avian Pneumovirus Vaccine; Phylogenetic Analysis of Neuraminidase Gene of Avian Influenza A Viruses
These researchers were involved in two projects using MSI resources during this period. In the first, they are developing a second-generation recombinant vaccine for avian pneumovirus (APV) infections in turkeys. They are using an infectious molecular clone of APV-C and are introducing mutations in this clone that will attenuate the virus. They are also creating mutations that serve as markers to distinguish the vaccine from the virulent strain.
The second project investigates the neuraminidase (NA) gene of avian influenza A viruses isolated from wild birds. The gene is amplified and sequenced using subtype-specific primers. Sequences are aligned using the Sequencher program to generate full-length sequences, which are then annotated. The researchers use MEGA software for phylogenetic analysis of the genes. They have designed primers specific for each N-sybtype using DNAStar software. These primers are used for amplification and sequencing of the NA gene from each subtype.
The group is using the BSCL, SDML, and SDVL for these projects.
Yogesh Chander, Research Associate
C. Ashok Kumar, Research Associate