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Research Abstracts Online
January 2009 - March 2010

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Genetics, Cell Biology, and Development

PI: Perry B. Hackett

Sleeping Beauty Transposon Studies

The goal of this project is to increase the utility of the Sleeping Beauty transposon system in all its applications, especially human gene therapy. The SB transposon system consists of two components, a transposon and a transposase enzyme that directs mobilization of the transposon from one DNA molecule to another. The reaction begins with the binding of SB transposase enzyme to its binding sites at the termini of the transposon. Currently the structure of SB transposase/transposon DNA complex is unavailable, but it is required for the further development of the SB transposon system. These researchers use NMR spectroscopy to obtain the structure of the complex. Molecular modeling and molecular dynamics simulations will be performend to assist with structural work and to gain information about the dynamics of SB transposase.

Group Member

Irina Nesmelova, Department of Physics and Optical Science, University of North Carolina, Charlotte, North Carolina