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Research Abstracts Online
January 2009 - March 2010

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Biochemistry, Molecular Biology, and Biophysics

PI: John D. Lipscomb, Associate Fellow

Structure and Mechanism of Oxygenase Enzymes

Oxygenase enzymes utilize molecular oxygen to oxidize a wide range of biological and manmade compounds with the incorporation of one or both atoms of oxygen into the products. These researchers are using the BSCL for two projects concerning these enzymes. In the first project, the researchers are studying a series of dioxygenase enzymes, which interact with aromatic compounds. This causes enormous amounts of carbon to reenter the carbon cycle and also allows manmade aromatics, some of which are carcinogens, to be degraded. In collaboration with the group of Douglas Ohlendorf, these researchers have solved the crystal structures of three of these enzymes. They are using the BSCL to examine the crystal structures and plan site directed mutagenesis studies, which are currently ongoing. Recently, the group has been able to structurally characterize the first reaction cycle intermediates by carrying out the reaction in a crystal and analyzing the data using the BSCL.

The second class of oxygenase being studied is typified by methane monooxygenase. This enzyme catalyzes the oxidation of methane to methanol with the incorporation of one atom of oxygen. Methane is generated in large quantities in the environment and is a potent greenhouse gas. It is prevented from reaching the atmosphere by the action of methane monooxygenase. Again in collaboration with Professor Ohlendorf, the group has solved the crystal structure of the critical hydroxylase component and is using the BSCL to visualize the structure and plan mutagenesis studies.

Group Members

Rahul Banerjee, Graduate Student
Sarmistha Chakrabarty, Graduate Student
Elena G. Kovaleva, Research Associate
Thomas M. Makris, Research Associate
Michael Mbughuni, Graduate Student
Vincent M. Purpero, Research Associate
Melanie Rogers, Research Associate