Research Abstracts Online
January 2009 - March 2010
University of Minnesota Twin Cities
College of Biological Sciences
Department Biochemistry, Molecular Biology, and Biophysics
PI: Lincoln R. Potter
Identification of the Natriuretic Peptide Receptor A (NPR-A) Kinase
This project seeks to identify the cognate kinase of natriuretic peptide receptor A (NPR-A). The researchers are utilizing several different techniques to identify the NPR-A kinase. The techniques to identify the kinase in question include affinity purification of the kinase, in-gel kinase assays, and highly specific chemical crosslinking to NPR-A. The identification of interacting proteins from the above methods will be assessed by mass spectrometric analysis, such as peptide mass fingerprinting by MALDI-TOF-MS.
By utilizing these methods, the researchers hope to identify the cognate kinase of NPR-A. It is hoped that identifying the kinase will yield a pharmacologic target that can bolster NPR-A activity, which is often diminished in disease states such as congestive heart failure. Completion of this goal will advance our knowledge of a critical regulator of the cardiovascular system.
Andrea Yoder, Graduate Student