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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
Medical School
Department of Ophthalmology

PI: Deborah A. Ferrington

Proteomic Analysis of Retina Proteins With Aging, Disease, and in Immunoproteasome Knockout Mice

These researchers investigated the retinal proteome to distinguish protein changes that occur with aging and disease in human donor eyes and in rodents, and in mice with specific proteins genetically knocked out. This work involved answering research questions from this laboratory and collaborators from outside the University of Minnesota. The general approach was to utilize two-dimensional gel electrophoresis to obtain protein separation, followed by gel densitometry to distinguish changes in protein expression. Protein spots exhibiting altered expression are excised from the gel and digested with trypsin. Peptides that are eluted from the gel are used to identify the protein by mass spectrometry. The group used both MALDI-TOF and LTQ Orbitrap mass spectrometers. Software used for analysis includes Protein Pilot, Scaffold, and ProTIP.

Group Members

Rebecca Kapphahn, Staff
Pabalu Karunadharma, Graduate Student