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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
Medical School
Department of Pediatrics

PI: Cheryl A. Gale

Quantitative Fluorescence Microscopy of Candida albicans Hyphal Tip Structures

The highly polarized morphology of Candida albicans hyphae and the directionality of hyphal growth are dependent on the Spitzenkörper. Strains lacking the GTPase Rsr1, or its GTPase-activating protein (GAP), have defective hyphal morphology and growth tropisms. This group has hypothesized that Rsr1 cycling is important for Spitzenkörper function. The Rsr1 GAP, Bud2, and guanine nucleotide exchange factor (GEF) Bud5 are differentially localized in hyphal tips. To determine if Rsr1 cycling is required for Spitzenkörper function, these researchers used time-lapse microscopy to follow a fluorescently tagged version of a Spitzenkörper-associated protein. Quantization of time-lapse fluorescent videos showed that strains lacking Rsr1, Bud5, and Bud2 showed an altered fluorescence distribution at septation. This data supports a model of Rsr1 cycling at the hyphal tip that is required for maintenance of the Spitzenkörper at specific times during the cell cycle.

Group Members

Timothy Heisel, Staff
Sara Kegley, Staff
Rebecca Pulver, Graduate Student
Kendra Van Buesekom, Staff