Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
College of Veterinary Medicine
of Veterinary Population Medicine
PI: Sagar M. Goyal
Phylogenetic Analysis of Neuraminidase Gene of Avian Influenza A Viruses; Poult Enteritis Leads to Light Turkey Syndrome
These researchers used MSI resources for two projects during this period. In the first, the neuraminidase (NA) gene of avian influenza A viruses, isolated from wild birds, was amplified and sequenced using subtype-specific primers. Sequences were aligned using the Sequencher program to generate full-length sequences, which are then annotated. The researchers used MEGA software for phylogenetic analysis of the genes. They have designed primers specific for each N-subtype using DNAStar software. These primers are used for amplification and sequencing of the NA gene from each subtype. The group used the BSCL, SDML, and SDVL for this project.
A new project begun during this period is aimed towards understanding causes of Light Turkey Syndrome (LTS). The researchers are detecting viruses—astrovirus, rotavirus, reovirus, and coronavirus—in the fecal samples collected from turkeys on LTS and non-LTS turkey farms. For the detection of viruses, they are developing an RT-PCR method using specific primers. They design the primers using DNAStar. Amplified sequences are aligned using Sequencher and phylogenetic analysis is done using MEGA. These resources can be found at the BSCL, CGL, SDML, and SDVL.
Yogesh Chander, Research Associate