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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Genetics, Cell Biology, and Development

PI: Perry B. Hackett

Structural Studies of Sleeping Beauty Transposon

These researchers have initiated a project related to structural studies of the Sleeping Beauty (SB) transposon system. The SB transposon system consists of two components, a transposon and a transposase enzyme that directs mobilization of the transposon from one DNA molecule to another. The reaction begins with the binding of the SB transposase enzyme to its binding sites at the termini of the transposon. Currently the structure of the SB transposase/transposon DNA complex is unavailable. The SB transposon system is being developed for genetic applications, including gene therapy. Structural information on SB transposase/transposon DNA complex is required for rationalizing efforts on improving the activity of the SB transposon system. The researchers use NMR spectroscopy to obtain the structure of the complex. They also use homology modeling and MD simulations to assist with structural work and to gain information about the dynamics of SB transposase.

Group Members

Irina C. Nesmelova, Department of Physics and Optical Science, University of North Carolina, Charlotte, North Carolina