Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
College of Biological Sciences
of Genetics, Cell Biology, and Development
PI: Tom S. Hays
Studies on the Structure and Phosphoregulatin of Cytoplasmic Dynein
These researchers study the phosphoregulation of Drosophila cytoplasmic dynein and test the hypothesis that phosphorylation of subunits can regulate dynein targeting and function. Previous studies of dynein phosphorylation are limited, the identified sites are controversial, and no studies have been approached in a genetically tractable system where the function of sites can be tested. In collaboration with Dr. John Yates (Scripps Research Institute), these researchers are using mass spectrometry to analyze the sites of subunit phosphorylation in the Drosophila cytoplasmic dynein complex. Their priority is the identification of phosphorylation sites within the IC, LIC, and LC subunits that may function in cargo attachment.
The researchers use software at the BSCL, CGL, and SDVL for sequence analysis and comparisons.
Yungui He, Research Associate
Mingang Li, Research Associate