Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
PI: Chandy C. John
Gene Expression From PaxGene Blood Samples During Severe Malaria
Total RNA has been purified from blood samples taken from Ugandan study subjects. These study subjects were enrolled in the study as either community controls with no malarial infection or they had either of the two forms of severe malaria. The goal of the project is to determine differential gene expression during cerebral malaria and during severe malarial anemia. To date, 12 RNA samples have undergone gene expression analysis with the Illumina Whole-Genome Gene Expression Direct Hybridization Assay. The researchers are continuing to submit samples to the University of Minnesota’s BioMedical Genomics Center for microarray data. They are also using MSI software for microarray data analyses. Particularly, they use GeneSpring to analyze microarray data for significant differences in gene expression and for pathway analyses.
Jennifer C. Koziol Wozniak, Graduate Student
Gregory Park, Research Associate