Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
PI: Lorene M. Lanier
Restoration of Wide-Field Images
The Lanier lab uses wide-field (epi-fluorescence) imaging to study the role of cytoskeletal regulatory proteins during neuronal development and differentiation. They employ a combination of live-cell imaging of fluorescently tagged proteins and correlative-immunofluorescence of fixed specimens. Images are captured on a Zeiss Axiovert 200M using Openlab software. The researchers are particularly interested in determining the localization of specific proteins in dendritic spines, small (<2 μm) protrusions on the surface of neurons. Visualization of these structures are greatly enhanced by deconvolution using the Iterative Restoration module in the Velocity Software Package, which is available through the BSCL.
Rachel Penrod, Graduate Student