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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Genetics, Cell Biology, and Development

PI: David A. Largaespada

Using Insertional Mutagenesis Techniques for Cancer Gene Discovery and Functional Genomics in Transgenic Mouse Models

These researchers are working to exploit insertional mutagenesis for cancer gene discovery and functional genomics in the mouse. They use a vertebrate-active transposon system, called Sleeping Beauty (SB), for insertional mutagenesis in mouse somatic and germline cells, and for gene therapy. Using SB they have developed a powerful method to find new cancer genes using transgenic mouse models. This approach can be used to understand the genetic basis of many types of cancer, including brain tumors, carcinomas of the liver and gastrointestinal tract, leukemias, sarcomas, and many more. Insertional mutagenesis is also being used to identify genes and genetic pathways underlying acute myeloid leukemia (AML) development. Ongoing work includes genetic studies of myeloid leukemia chemotherapy resistance and RAS pathway oncogene addiction.

Analysis of data involves the development of custom programs, as well as the use of packaged software, such as Ingenuity and GeneData Expressionist.

Group Members

Raha Allaei, Graduate Student
Bridget S. Charbonneau, Undergraduate Student
Caitlin Conboy, Graduate Student
Craig Eckfeldt, Graduate Student
Julia Hatler, Research Associate
Vincent Keng, Research Associate
Jon D. Larson, Graduate Student
Branden Moriarty, Graduate Student
Charles R. Paule, Staff
Eric Rahrmann, Graduate Student
Sue Rathe, Graduate Student
Zohar Sachs, Research Associate
Aaron Sarver, Research Associate
Timothy Starr, Research Associate
Adrienne Watson, Graduate Student