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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
College of Veterinary Medicine
Department of Veterinary Biomedical Sciences

PI: K.V. Nagaraja

Characterization of Clostridium perfringens Type A Causing Necrotic Enteritis and Dermatitis/Cellulitis in Poultry Through Genomic and Proteomic Approaches

Concern regarding the incidence of clostridial diseases in poultry production has grown in the last few years. One of the most commonly observed organisms responsible is the anaerobic, spore-forming bacteria Clostridium perfringens type A. In poultry, it has been cultured from the lesions of birds (broiler chickens) with gangrenous dermatitis and is identified as the organism responsible for the pathology associated with necrotic enteritis in broilers as well as cellulitis in turkeys. All the three disease conditions appear distinct and no single vaccine is found to be effective against all the three. Little is known about the genetic as well as phenotypic relationship between C. perfringens type A isolates cultured from gangrenous dermatitis/cellulitis and necrotic enteritis. The objective of this study is to determine the genetic variations among C. perfringens isolates from cases of cellulitis in turkeys in comparison to isolates from other disease conditions. The genetic relationship of all these C. perfringens isolates will be determined using multi-locus sequence typing (MLST). The sequences are analyzed using software such as DNA Star and Sequencher, and the phylogenetic relationship is analyzed. The results of this study will throw light into the genotypic characters of each C. perfringens type A causing different disease conditions in poultry.

Group Member

Anil Thachil, Research Associate