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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Duluth
College of Pharmacy Duluth
Department of Pharmacy Practice and Pharmaceutical Sciences

PI: Jon N. Rumbley

Protein-Protein and Protein-Substrate Specificity in Two Unique Membrane Bound Proteins

This researcher is working on the characterization of protein-protein and protein-substrate recognition/specificity by sweet taste receptors and organic anion transporting polypeptides (Oatps), respectively. In each case, the protein structures have been constructed by homology modeling for use in structure based hypothesis generation. Of interest for the sweet taste receptor is the extracellular hydrophilic domain and its interaction with both small ligands and, more importantly, with the sweet-tasting protein brazzein. Protein-protein docking and molecular dynamics are used to understand how brazzein binds and elicits its sweet taste, with interest in surface complimentarity and backbone flexibility. The computational work is being used to drive mutagenesis studies on brazzein.

The Oatp project is concerned with small-molecule transport selectivity across the cell membrane. Oatps appear to be members of the large Major Facilitator Superfamily (MFS) of transporters, which are used for modeling. The researcher is using small molecule docking and dynamics, pharmacophore modeling, and 3D-QSAR.