Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
of Laboratory Medicine and Pathology
PI: Yoji Shimizu
The Role of Intercellular Adhesion Molecule-1 (ICAM-1) on CD8+ T-Cells During Their Differentiation Into Effector and Memory T-Cells
This project focuses on the factors that drive the differentiation of CD8+ T-cells into cytotoxic T-lymphocytes and long-lived memory T-cells. The means by which T-cells acquire effector and memory status upon activation is a topic of significant interest in issues ranging from anti-tumor therapeutics to vaccine development. These researchers are interested in the role of the integrin binding adhesion molecule, ICAM-1, on CD8+ T-cells. They aim to understand the mechanism by which ICAM-1 signaling controls T cell differentiation by using gene expression profiling to identify the genes regulated by ICAM-1 during T cell differentiation. They also plan to reconstitute ICAM-1-/- T-cells with recombinant virus encoding ICAM-1 mutants and observe their ability to "rescue” WT function. In vivo adoptive transfer studies will deduce the role of ICAM-1 in forming memory precursor and short-lived effector T-cells. This research is important because it describes novel regulatory functions of T-cell expressed ICAM-1 that can help define the characteristics for proper CD8+ T-cell differentiation upon activation.
Nicholas Zumwalde, Graduate Student