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Research Abstracts Online
January 2010 - March 2011

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University of Minnesota Twin Cities
Academic Health Center
Masonic Cancer Center

PI: Natalia Y. Tretyakova

Effects of Cytosine Analogs on DNA Adduct Formation

The reactivity of tobacco carcinogens towards guanines in DNA is affected by the presence of 5-methylcytosine base paired to the reacting guanine. To further investigate this result, these researchers incorporated various 5-methylcytosine analogs into duplex oligonucleotides and determined the experimental reactivity data. Quantum mechanical calculations were then used to investigate the properties of these 5-substituted cytosines in order to rationalize the experimental results. The calculations were also used to identify other key cytosine analogs that could be used to probe the effect of 5-substituted cytosine on guanine reactivity with tobacco carcinogens. The researchers are performing further quantum mechanical and molecular dynamic calculations to investigate base pairing effects and DNA duplex structure and reactivity. Continued studies will involve dynamic simulations of structurally modified dC analogs within short DNA duplexes and their complexes with BPDE. These studies will analyze the effects of substitution at C-5 of dC on DNA duplex structure and possible transition state geometries for BPDE reaction with DNA.

Group Members

Darrin M. York, Fellow, Co-Principal Investigator
Thakshila D. Dissanayake Rallage, Graduate Student
Delshanee Kotandeniya, Research Associate
Elbek K. Kurbanov, Staff
Xun Ming, Graduate Student