Research Abstracts Online
January 2010 - March 2011
University of Minnesota Twin Cities
Office of the Vice President for Research
PI: Jinhui Zhang
Different Changes of Proteome Profiles in the Dorsal-Lateral and Ventral Prostate of TRAMP Mice
The transgenic adenocarcinoma of mouse prostate (TRAMP) model is being widely used for screening of chemo-therapeutic/preventive agents against prostate cancer. However, the molecular mechanisms of the two lobe-specific lineage of carcinogenesis are still not very well defined. Here, for the first time, these researchers concurrently profiled the proteome of dorsal-lateral (DLP) and ventral (VP) lobes from the prostate of both TRAMP and littermate wild-type C57BL/6 mice at the age of 18 weeks by 2D LC-MALDI-TOF/TOF with iTRAQ labeling. In total, 483 and 748 proteins were identified at critical false discovery rates of 1% and 5%, respectively. In wild-type mice, 84 proteins were found to have different expression levels between DLP and VP such as experimental autoimmune prostatitis antigen 2 (EAPA2), beta-tropomyosin, and clusterin. In TRAMP mice, a total of 118 proteins were found to be significantly changed in DLP and/or VP during TRAMP carcinogenesis. Among these 118 proteins, 55 and 36 proteins were uniquely changed in the DLP or VP lobe, respectively, and 27 proteins in both DLP and LP lobe. Ingenuity Pathway Analysis was able to segregate proteins changed in two lobes into different pathway networks. Overall these datasets highlighted that different mechanisms were involved in the carcinogenesis in DLP and VP. Further functional studies and bioinformatics analysis on those differentially expressed proteins will help the researchers narrow down the key mediator(s) of carcinogenesis and lobe-specific prostatic development.
Li Li, Research Associate