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Research Abstracts Online
January - December 2011

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Genetics, Cell Biology, and Development

PI: Vivian J. Bardwell

DMRT1 in Mammalian Sexual Development

DMRT1 is a key regulator of testicular development, controlling germ cell and Sertoli cell differentiation. To pinpoint the biological and biochemical processes directly controlled by DMRT1 during testis development, the genes that DMRT1 directly regulates must be identified. These researchers have used chromatin immunoprecipitation coupled to Next Generation Sequencing (ChIP-Seq) to identify promoters bound by DMRT1 and proteomics to identify interacting partners for DMRT1. They routinely use computation methods to analyze raw data and to functionally annotate the DMRT1 target genes and interacting partners. This analysis will highlight genes encoding potential regulatory proteins and may help find regulatory circuits controlled by DMRT1. This project uses several MSI resources, including processing raw data on the Linux clusters, the Galaxy server for analyzing data, the Tropix server for analyzing mass spectrometry data, and the project space for storing large NGS and mass spectrometry datasets.

Group Members

Micah Gearhart, Research Associate
Tony Krentz, Graduate Student
Mary Kroetz, Research Associate
Mark W. Murphy, Research Associate
Aaron Sarver, Research Associate
David A. Zarkower, Faculty Collaborator