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HuWS

Research Abstracts Online
January - December 2011

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University of Minnesota Twin Cities
College of Science and Engineering
Department of Chemical Engineering and Materials Science

PI: Wei-Shou Hu, Associate Fellow

Genomic Explorations in Biotechnology

These researchers are involved in two projects using MSI resources. In one, they are using genomics and proteomics to investigate recombinant Chinese hamster ovary cells. These industrially important cells carry many desirable phenotypic traits, but the alterations at the genomic level that confer those traits are not well understood. The researchers are seeking a better understanding of those key genetic alterations, which will facilitate the development of new cells lines and new processes.

A second project uses data mining to analyze cell-culture manufacturing processes. The comprehensive data archives of these modern plants present vast underutilized resources containing information that, if unearthed, may enhance process robustness. Process data of about fifty production “trains,” comprising electronic logs of material input, temporal on-line and off-line measurements of reactor state variables, operational parameters, and key metabolites and nutrients, have been analyzed. Pattern recognition techniques including kernel-based support vector machines were employed to elucidate significant correlations between process variables and the outcome. Importantly, interpretation of these predictive models through expert analysis provided further insights about physiological variables that affect productivity. Such data-mining efforts will aid in developing effective control strategies to ensure process consistency and high productivity.

Group Members

Sami Al-Marzoog, Undergraduate Student
David Bulnes, Tecnológico de Monterrey, Mexico
Marlene Castro-Melchor, Graduate Student
Anushree Chatterjee, Graduate Student
Dong Seong Cho, Graduate Student
Yuqing Cui, Undergraduate Student
Nitya M. Jacob, Graduate Student
Kathryn Johnson, Graduate Student
Edna Justiniano, University of Puerto Rico, Puerto Rico
Huong T. Le, Graduate Student
Terk Shuen Lee, Bioprocessing Technology Center, National University of Singapore, Singapore