Research Abstracts Online
January - December 2011
University of Minnesota Twin Cities
PI: Theresa A. Laguna
Biomarkers of Lung Injury and Remodeling in Cystic Fibrosis
Cystic fibrosis lung disease is characterized by structural damage to the airways and parenchyma. Chronic inflammation and infection lead to airway injury and remodeling, causing loss of lung function and shortened life expectancy. The non-invasive measurement of desmosine concentration in urine and sputum may be reflective of chronic and acute lung injury. The hypothesis of this project is that the concentration of desmosine in urine and sputum will increase from values obtained during an immediately prior stable period in patients experiencing a pulmonary exacerbation, and will correlate with previously validated measures of lung disease, including inflammatory markers and lung function. The aims of the project are: to determine natural change and variability of desmosine concentration in a cohort of CF patients during times of clinical stability and during pulmonary exacerbation; to determine if desmosine concentration can differentiate between a clinically stable and exacerbated state; and to identify new potential biomarkers through novel metabolomic and proteomic techniques. Determination of airway desmosine concentration plus the identification of new biomarkers may allow for the development of a panel of biomarkers to facilitate early medical intervention to help preserve lung function. Proteomics and metabolomics methods are used to analyze CF urine and sputum to detect and measure desmosine as well as to determine the proteomic/metabolomic profile of different phenotypes of CF patients.