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PanoskaltsisMortariA

Research Abstracts Online
January - December 2011

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University of Minnesota Twin Cities
Medical School
Department of Pediatrics

PI: Angela Panoskaltsis-Mortari

The Role of Lung Matrix in Health and Fibrotic Disease

The extracellular matrix (ECM) is a target of remodeling during lung diseases such as idiopathic pulmonary fibrosis and broncholitis obliterans. This group’s research focuses on how the mechanical properties, structure, and lung function change during different manifestations of lung disease. Using a mouse model of different diseases, the researchers are able to perform pulmonary function tests, examine organ histology, run tests of biaxial tensile strength, and examine the three-dimensional structure of the lung by using 2-photon microscopy, which then provides a full picture of how the lung is impacted after injury and/or disease. During biaxial tension testing, a high-speed camera records a labeled sample while load cells record forces, allowing for strain mapping and basic quantification of the mechanical properties of the lung. Strain mapping analysis requires image processing, mesh generation in Abaqus, and particle tracking in MATLAB, all of which are done using MSI resources. Collagen and elastin are main components of the lung ECM, and both have been imaged with 2-photon microscopy, which can be processed to generate a 3D map of the ECM structure, and other pertinent MSI resources are used for this image processing and analysis. Combining the mechanical testing and 2-photon imaging techniques with established pulmonary function and histology data will allow for development of a more comprehensive picture of lung disease.

Group Members

Alex P. Domek, Undergraduate Student
Lindsay Godin, Research Associate
Andy Price, Staff
Paige Voigt, Undergraduate Student