Research Abstracts Online
January - December 2011
University of Minnesota Twin Cities
of Laboratory Medicine and Pathology
PI: Yoji Shimizu
Microarray Analysis of T Cell Activation
This group’s research is focused on understanding the biological activation of the adaptive immune system. In particular, they are investigating how the adhesiveness of white blood cells, including T lymphocytes, controls priming and responsiveness to new infections. This information is important not only from a basic perspective but also in the development of new vaccine strategies for combating infection and for tumor immunotherapy.
Two specific projects ongoing in the laboratory are aimed and deciphering molecular mechanisms underlying the function of the cell surface ICAM-1 receptor and the intracellular ADAP adapter proteins. Both of these proteins are important for T cell adhesiveness and thus represent important components during the initial stages of T cell activation. The researchers have initiated RNA microarray studies using cells from mice that are deficient in ICAM-1 or ADAP. In concert with these genetic systems, they are using MSI resources, including the Agilent GeneSpring software package, to analyze and validate their microarray data.
Brandon J. Burbach, Research Associate
Nicholas Zumwalde, Graduate Student