Research Abstracts Online
January - December 2011
University of Minnesota Twin Cities
PI: Martin W. Wessendorf
Heterodimerization of Opioid Receptors
Pairs of different g-protein coupled receptors have been reported to heterodimerize. By doing so, they should exist within a few nanometers of each other. If these receptors do in fact heterodimerize, it should be possible to use light microscopy to detect single structures in the central nervous system that are labeled for both receptors.
These researchers have been able to detect single structures at the limit of resolution by confocal microscopy (i.e., smaller than 0.3 mm) that are labeled for two different types of receptors. Resolution could in principle be improved by deconvolving the confocal images, however. The researchers use the MSI’s laboratory resources to deconvolve such images. They anticipate at least a two-fold improvement in resolution by doing so. Obtaining higher-resolution images improves the ability to test which receptors, if any, may exist as heterodimers and to determine their distributions. In addition, the researchers are using MSI software to design probes that will allow them to localize various receptors using immunocytochemistry and/or in situ hybridization.
Mai Lan Leong, Graduate Student
Steve Schnell, Staff