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WilmotCM

Research Abstracts Online
January - December 2011

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University of Minnesota Twin Cities
College of Biological Sciences
Medical School
Department of Biochemistry, Molecular Biology, and Biophysics

PI: Carrie M. Wilmot, Associate Fellow

Molecular Mechanisms of Amino Acid-Derived or Metal Cofactors in Proteins: Their Function and Assembly

The goal of this project is to understand the synthesis and function of novel organic, organometallic, and metal ion cofactors in proteins. The principal tool of the research is macromolecular x-ray crystallography. The project involves freeze-trapping reaction intermediates in the crystal to yield molecular “snapshots” along the reaction pathways through interrogation by x-rays. These can be assembled into “movies of catalysis” at the molecular level. By understanding these reactions in such detail, it is hoped that better drugs can be designed, proteins can be rationally engineered for biotechnological purposes, and chemists can design simpler industrial catalysts to control analogous reactions. Specific topics of interest to the Wilmot lab include structural enzymology involving hemes (chlorite dismutase, MauG, hydroxylamine oxidoreductase), assembly of the cofactor tryptophan tryptophylquinone of methylamine dehydrogenase, and molecular oxygen activation by the copper-containing amine oxidase from yeast.

Group Members

Christopher Carrell, Research Associate
Peder Cedervall, Graduate Student
Brandon Goblirsch, Graduate Student
Lyndal Hill, Research Associate
Ed Hoeffner, Staff
Val Klema, Graduate Student
Jackie L. McCourt, Graduate Student
Corinne Solheid, Undergraduate Student
Erik T. Yukl, Research Associate