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analysis colorectal cancer genes
Analysis of Candidate Colorectal Cancer Genes
Colorectal cancer (CRC) is the second leading cause of cancer death in the US. CRC develops through progressive accumulation of somatic genetic and epigenetic alterations. Though many CRC mutations have been characterized, a significant percentage of genetic changes remain to be identified and their function in cancer understood. The long term goal of this research is to identify and characterize these alterations in order to better understand the pathogenesis of this cancer and to develop therapeutic and diagnostic tools to attack it. Genetic screens using the Sleeping Beauty transposon mutagenesis system have identified over 100 candidate cancer genes in a mouse model of colon cancer. The researchers are currently studying the role of the most promising of these candidates using mouse models, intestinal epithelial cell lines, and organoid cultures. As part of these studies they are conducting global analyses of gene expression using bead arrays and RNA sequencing. They use MSI resources for help in analyzing these expression studies.