Effect of PO2 on Protein Translation in Alveolar Epithelial Cells
The alveolar epithelium is exposed to a broad range of PO2 levels during development, disease and treatment with mechanical ventilation. The epithelial cell responses to injury are critical to understand in order to promote lung growth and repair. This project examines the effect of hypoxia and hyperoxia on the translation of proteins by alveolar epithelial cells exposed to altered PO2 levels in vitro or in vivo. Polyribosome preparations are made to assess which mRNAs are being translated in large and small amounts. mRNA isolated from the heavy (high translation) fractions and total ribosome-bound mRNA are used in gene array experiments comparing the mRNA distribution with different ambient PO2 concentrations. MSI resources are used for data acquisition and analysis. The underlying hypothesis is that there are sets of proteins whose translation is specifically up or down regulated with the stresses of hyperoxia and hypoxia. The researchers anticipate that some sets will move up or down similarly with hyperoxia and hypoxia, whereas other sets will have differentiated responses to these two conditions.