Microarray Analysis of T Cell Activation
This group’s research is focused on understanding how the adhesiveness of white blood cells including T lymphocytes controls immunological responses to new infections. In general, these studies seek to provide novel information on the mechanisms by which integrins and integrin counter-receptors contribute to efficient and specific immunity. In particular, the researchers intend to identify molecular targets for the modulation of immune responses, including the optimization of cell culture conditions for the generation of tumor-specific T cells effective in anti-cancer immunotherapies. This information is important not only from a basic perspective but also in the development of new vaccine strategies for combating infection and for tumor immunotherapy.
Two specific projects ongoing in the laboratory are aimed and deciphering molecular mechanisms underlying the function of the cell surface ICAM-1 receptor and the intracellular ADAP adapter proteins. Both of these proteins are important for T cell adhesiveness and thus represent important components during the initial stages of T cell activation. The researchers have initiated RNA microarray studies using cells from mice that are deficient in ICAM-1 or ADAP. In concert with these genetic systems, they are using MSI resources, including the Agilent GeneSpring software package, to analyze and validate their microarray data.