Soft Targeting Safe Havens for Sleeping Beauty (SB) Transposon Insertion Using RecA

Abstract: 

Soft Targeting Safe Havens for Sleeping Beauty (SB) Transposon Insertion Using RecA

The goal of this project is to demonstrate the efficacy of Sleeping Beauty (SB) transposons as vectors for non-viral gene delivery to treat mucopolysaccharidosis diseases. A specific goal for development of the SB system for humans is to maximize the safety of using transposons that randomly integrate into genomes by directing their integration to sites that are thought to be safe. Certain repetitive elements meet this criterion. These researchers will be infusing SB transposons with targeting reagents into mouse liver genomes. They then will harvest the DNA from the liver cells, label the restricted DNA with "tags" and submit the DNA fragments for high-throughput sequencing. They will be looking for association of SB sequences juxtaposed to repetitive element sequences. The project involves massive numbers of DNA sequences. The researchers will use MSI resources to interpret the data and, hopefully, validate the model. MSI resources such as Galaxy are particularly useful when assembling and interpreting DNA sequence data from LUMINA runs.

Group name: 
hackettp