Extracellular vesicles (EVs) are small membranous structures that are between 50 and 1000 nm in diameter and are present in extracellular spaces and bodily fluids. They are formed from cells in vivo and in vitro from either exocytosis of endosomal vesicles or from plasma membrane blebbing. While initial observations assumed the EVs were cellular waste or debris, evidence now indicates that EVs are part of a signaling mechanism that conducts molecular messages between cells either locally or remotely (long distances). Major signaling molecules in EVs are micro RNAs and mRNAs. In this study EVs are isolated from animal blood or cultured cell media. The isolated EVs are than extracted to collect their total RNA content. The total RNA is used to construct a library and the library is subjected to next generation sequencing. The reads will be quantified, annotated, and analyzed.