These researchers are investigating the role of Ras signaling pathways in acute myeloid leukemia using mouse and human AML model systems. They have generated RNA sequencing data comparing NRAS oncogene-dependent mouse AML samples with "relapsed" NRAS oncogene-independent derivative AMLs that are undergoing bioinformatic analysis. They use MSI resources to analyze gene expression and network analyses (i.e. IPA), as well as look for fusion genes, alternate splice forms, and single nucleotide variations. The researchers are working to complete additional related WES and/or RNA-sequencing projects in the next year to build on their findings. They are also beginning to perform larger scale proteomic analysis with mass cytometry and reverse phase protein array (RPPA). Overall, the goal of this work is to characterize RAS signaling in AML growth and survival, and guide the development of novel targeted treatment approaches for AML patients.