Professor Michael Mauer MD

Medicine
Medical School
Twin Cities
Project Title: 
Microarray and RNA-seq Studies of Skin Fibroblasts in Type 1 Diabetes Diabetic Nephropathy

The Mauer laboratory is continuing with gene expression research studies using microarray and RNA-seq methods. Previous work has included analysis of the Affymetrix microarray data from pairs of MZ Twins discordant for type 1 diabetes (T1D). The group has also run Illumina RNA-seq assays on nine pairs of MZ Twins discordant T1D under high and low glucose conditions, using MSI resources such as Galaxy and additional genomic software programs to analyze this data. They also have RNA-seq mRNA sequencing data from University of Minnesota Genomics Center Core facility on 100 human skin fibroblast and 100 LCL samples from a JDRF (GoKind) diabetic cohort, and have gone through an initial analysis. The 100 skin fibroblast and LCL RNA-seq data has gone through an initial analysis as the researchers continue to try and understand the data and prepare it for submission for publication. In addition, preliminary microarray experiments have been run on the nine twin pairs for miRNAs which, can act as negative regulators of gene expression. The goal is to eventually run these miRNA samples using the Illumina small RNAseq assay. The researchers are using MSI's computer and software resources.

Project Investigators

Associate Professor Luiza Caramori
Yong Hwan Han
Professor Youngki Kim
Professor Michael Mauer MD
 
Are you a member of this group? Log in to see more information.