Office of VP for Research
Hepatic stellate cells (HSCs) seeded on a stiff matrix display a higher level of p300 acetyltransferase that is accumulated in the nucleus. Becasue p300 is a transcription coactivator for many transcription factors, these researchers hypothesize that many genes may be turned on for transcription in cells grown on a stiff matrix due to the upregulation of p300. Therefore, they are using RNA-seq to analyze gene expression to identify genes that are turned on by a stiff matrx.
To this end, three HSCs samples grown on 400 Pa and three HSCs grown on 25,600 Pa will be subjected to RNA isolation. Samples will be sent to the University of Minnesota Genomics Center center for RNA sequencing and the data will be compared between two groups using software available at MSI. MSI storage resources will also be used for the data.