Office of VP for Research
Cultured primary human hepatic stellate cells (HSCs) were preincubated with FAK1 inhibitor PF228 (5 uM) and stimulated with TGF-beta1 for 24 hours. Cells were collected for RNA isolation. RNA samples were then sent to the University of Minnesota Genomics Center for next generation RNA sequencing. There are four groups under study in this project: HSC, HSC+TGF-beta1, HSC+PF228 and HSC+PF228+TGF-beta1.