Mycobacterium tuberculosis, the causative agent of tuberculosis, remains a leading cause of death by infectious diseases globally. One of the hallmarks of tuberculosis is the ability of the bacteria to cause persistent infection by evading the host adaptive immune response. The Tischler lab seeks to understand the bacterial factors that enable Mycobacterium tuberculosis to persist. They use a combination of bacterial genetics and a mouse model of tuberculosis infection to identify and characterize these persistence mechanisms. This work is currently focused on a bacterial phosphate-sensing signal transduction system that is necessary for persistence. The researchers are also currently conducting high-throughput genetic screens using Tn-seq technology to identify novel persistence mechanisms as well as mechanisms that enable the bacteria to tolerate treatment with antibiotics.