Tn-Seq: Microbial Genetics for the 21st Century and PacBio Genomes
When a saturating transposon library (~ 100,000 mutants) is generated in an organism, changes in the frequency of each insertion mutant during selection can be determined by sequencing flanking genomic regions en masse. The frequency of each insertion is used to determine a fitness score. Using this method, the fitness for each gene in an entire genome for any given condition can be determined in one experiment. Tn-seq eliminates inherent biases common to transcriptional studies and regulation is taken out of the equation all together. The transposon used in these studies has also been shown to produce saturation libraries in a range of other model organisms throughout the Bacteria and Archaea. These researchers use MSI resources to analyze and compile Illumina sequencing data. They have also begun sequencing bacterial genomes using PacBio and plan to use MSI facilities for analysis.
A bibliography of this group’s publications is attached.
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