Project abstract for group largaesp

Using Insertional Mutagenesis Techniques for Cancer Gene Discovery and Functional Genomics in Transgenic Mouse Models

These researchers are working to exploit insertional mutagenesis for cancer gene discovery and functional genomics in the mouse. They use a vertebrate-active transposon system, called Sleeping Beauty (SB), for insertional mutagenesis in mouse somatic and germline cells, and for gene therapy. Using SB they have developed a powerful method to find new cancer genes using transgenic mouse models. This approach can be used to understand the genetic basis of many types of cancer, including brain tumors, carcinomas of the liver and gastrointestinal tract, leukemias, sarcomas, and many more. Insertional mutagenesis is also being used to identify genes and genetic pathways underlying acute myeloid leukemia (AML) development. Ongoing work includes genetic studies of myeloid leukemia chemotherapy resistance and RAS pathway oncogene addiction. Microarray and RNA-sequencing are techniques used to evaluate transcriptomes in the cancer models. Analysis of data involves the use of the Galaxy Suite, the development of custom programs, and the use of packaged software, such as the Galaxy RNA-seq toolkit, Ingenuity and GeneData Expressionist. 

A bibliography of this group’s publications acknowledging MSI is attached.