Project abstract for group manskylm

Molecular Biology of HIV and HTLV

These researchers are studying the molecular biology of human retroviruses. In one project, they investigate questions related to HTLV-1 particle size, Gag stoichiometry in particles, and HTLV-1 Gag interactions in living cells using multiple experimental approaches. In particular, they apply cryo-electron microscopy/tomography (cryo-EM/ET), total internal reflection fluorescence (TIRF) microscopy, and the novel single-molecule technology of fluorescence fluctuation spectroscopy (FFS) to investigate questions related to particle size and Gag stoichiometry, Gag targeting to membrane, and HTLV-1 particle biogenesis. A second project will conduct discovery studies to identify new small molecules that increase RT-mediated errors, use molecular analyses to identify the mechanism(s) by which small molecules increase the HIV mutation rate and result in virus extinction, and assess the mechanism of RT-mediated mutation using biochemical methods.