Structural Studies of APOBEC3 Proteins Using NMR
Human APOBEC3G (A3G) is capable of altering the HIV genome by deaminating cDNA cytosines to uracils. This activity can genetically inactivate the virus. As a counter-defense mechanism, HIV promotes the ubiquitin-mediated protein degradation of A3. Degradation requires that the A3 proteins interact with the HIV virion infectivity factor (Vif) protein. The major objectives of this research are to obtain a structural understanding of the DNA cytosine deaminase activity of A3G and of the A3G-Vif interaction. The researchers use NMR to solve protein structures, to study enzymatic kinetics, and to monitor protein-protein interaction. MSI resources are used to analyze NMR data.
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